Our Methods
Recombinant Protein Production
We use both E. coli and insect cells as host organisms to express our proteins of interest. We express small protein fragments for crystallisation, full length proteins, and protein complexes. We have the latest Akta FPLC systems with a full set of chromatography columns.
In vitro reconstituion
'What I cannot create, I do not understand'; Richard Feynmann's famous quote reflects one of the core principles of the lab. We reconstitute complexes and systems from purified recombinant material. By rebuilding macromolecular machines outside of the cell we have full control over the system, allowing us to study it in incredible detail.
X-ray crystallography
Using our purified proteins and complexes, we attempt to obtain high-resolution 'snapshots' of important domains and interactions within our proteins of interest.
Cryo-EM
Recent breakthroughs in direct electron detector technologies allow us to determine the high-resolution structure of even relatively small macromolecules. We have in-house access to vitrification robotics, and a new Talos Arctica microscope.
Yeast Genetics
2001: cell cycle
2006: transcription
2009: telomeres
2013: vesicle transport
2016: autophagy
Studies using yeast as a model organism have resulted in five Nobel Prizes in fifteen years. The incredible power, speed and tractability of yeast genetics will continue to serve science for years to come.
2006: transcription
2009: telomeres
2013: vesicle transport
2016: autophagy
Studies using yeast as a model organism have resulted in five Nobel Prizes in fifteen years. The incredible power, speed and tractability of yeast genetics will continue to serve science for years to come.
